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We examined the mechanism of the expression of the C-fos gene in quiescent 3T3-L1 preadipocytes. Expression of the C-fos gene is rapidly induced upon serum stimulation of quiescent cells. By using a polyclonal antibody that recognizes the phosphorylated form of p27Kip1, we examined the role of p27Kip1 in the regulation of the C-fos gene expression in 3T3-L1 preadipocytes. In 3T3-L1 preadipocytes, the C-fos gene was expressed within 2 h after serum stimulation and reached a maximum at approximately 6 h after the stimulation. The induction of the C-fos gene expression was accompanied by an increase in the amount of p27Kip1 phosphorylated in the nucleus. In contrast, in adipose cells from C3H mice that lack p27Kip1, the C-fos gene was not induced by serum stimulation. The presence of a major kinase activity that phosphorylates p27Kip1, which is stimulated by serum, was demonstrated. 0b46394aab
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